EFFECT OF SUCROSE,LIGHT INTENSTY AND AGRICULTURE MEDIUM ON ACCLIMATION OF DATE PALM (Phoenix dactylifera L .cv.Barhee)
Basrah Journal For Date Palm Research,
Volume 7, Issue 2, Pages 17-31
AbstractThe present study was undertaken at tissue culture laboratory/Date Palm Research Center-Basrah university to determine the effect of some treatments on the Acclimation of date palm plantlets cultivar Barhee propagated by tissue culture, a small plantlets produced by culturing a quarter of shoot tips obtained from date palm offshoots of Barhee were used in this study, the culturing were done on solid media composed of M.S minerals and some chemicals, in addition to activated charcoal, and 30Mg/L NAA and 3Mg/L 2ip.
The main results of this study were:
1-It was found that sucrose addition at a concentration of 60 gm/L to the media led to significant increase in the rooting percentage, roots number and length in comparison with the other tested concentrations 30 and 90 gm/L, the rooting percentage was 90%, the numbers of roots 4.2 per plantlet, while the length of roots was 5.8 cm.
2-The addition of sucrose at a concentration of 60 gm/L to the media led to significant increase of the average height of plantlets and the numbers of leaves per plantlet, the plantlet height reached 8.4 cm and the number of leaves was 2.2 per plantlet.
3-Light intensity had a significant role on the growth of date palm plantlets, the increasing of light intensity from 1000 lux to 3000 lux before one month of acclimation led to promote the growth parameters & the plantlets leaves were normal in their shape.
4-The sand and peatmoss medium (3:1) showed a high efficiency on the acclimatized plantlets percentage which reached 80%.
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