Effect of vitamin E on some callus and embryos characteristics of date palm cv.barhee propagated by in vitro
Basrah Journal For Date Palm Research,
Volume 9, Issue 2, Pages 1-12
AbstractThis study was conducted in the laboratory of tissue culture of the Palm Research Center in 2007 to find out the effect of adding different concentrations of vitamin E for the media and see its effect on the percentage of browning and growth of the Callus initial and the formation of embryos, were culturing on 100 mg of callus initial media container salts MS the forces of complete and sucrose 30 g / liter activated charcoal 3 g / l and plant growth of naphthalene acetic acid concentration of 30 mg / L and cytokines 2ip concentration of 3 mg / L and different concentrations of vitamin E (zero, 50,100,150 mg / L) and incubated explants under severe lighting 1000 lux and the temperature in 27±1, was carried out of agriculture every 4 weeks, and results showed the following:
1 - The addition of vitamin E for the media has reduced the percentage of browning of explants in difference significant comparison with treatment without vitamin D, as the percentage of browning 30% at a concentration of 100 mg / L compared with control treatment, which amounted to a rate of browning 90%.
2 - The addition of vitamin E for the media add to increase the percentage of the response of growth reaching 80.3% at a concentration of 100 mg / L, while the percentage reduced to 40% in the treatment comparison.
3 - increased rate of weight of embryo callus at a concentration of 100 mg / l as the rate of 280 mg, while decreased to 130 mg in the treatment of comparison, also helped add the vitamin to be vegetative embryos in less time as possible as it was 32 days at a concentration of 100 mg / liter significant difference from control treatment, which amounted to 52 days.
4 - The results showed that the addition of vitamin E concentration of 100 mg / L led to give the highest rate of vegetation as the number of embryos was 17 compared to the treatment comparison of embryos, which was the average number of embryos vegetative three embryos.
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