Isolation And Identification of Bacterial Types That Causes Contamination of Date Palm Phoenix dactylifera L Callus and studding Inhibitory activates of some plant extracts And Antibiotic
Basrah Journal For Date Palm Research,
Volume 10, Issue 1, Pages 68-82
AbstractThis research was conducted in Date palm research center, Basrah university- for isolation and identification of bacterial types that contaminated date palm tissue culture., and studied the inhibiting activities of three types of plant extracts on fruit of Rhus coriaria , bark of Cinnamomum zeylanicum and gummy extraction of Bswellia sp., using four types of solvent water, methyl alcohol, normal hexane and ethyl acetate, in three concentrations(0, 0.5, 1)% and studied the inhibitory activities of three types of antibiotics Gentamycin, Chloramphenicol and Amoxillin. The results of isolation and identification of bacteria appeared contanmination of callus tissue of date palm tissue culture by three genera of bacteria Staphylloccus aurwus , Bacillus subtillus and Proteus spp. The results showed that ethyl acetate extracts 1% of each Rhus coriaria and Bswellia sp. appeared the highest inhibition zone of about (23.00, 24.00)mm respectively against S. aurwus while the inhibition zone of ethyl acetate extract 1% of Bswellia sp. against
B. subtillus was18.33mm, the results showed that alcohol and ethyl for acetate Rhus coriaria extract were the best extracts that gave the highest inhibition zone(16.76, 16.33)mm respectively against Proteus spp.
The results of antibiotic inhibiting activates against bacteria genera appeared preeminence of Chloramphenicol 1% of about (28.67, 30.33. 29.00)mm of in habitation zone against Proteus spp. S. aurwus and B. subtillus respectively while Gentamycin 1% gave the higher inhibition zone of about 29.33 mmagianest Proteus spp in significant difference among other treatments.
- Article View: 49
- PDF Download: 2