Study of some of changes the growth of vegetation developing embryos under different levels of sucrose of the date palm Phoenix dactylifera L. var. Barhi.
Basrah Journal For Date Palm Research,
Volume 10, Issue 1, Pages 18-30
AbstractThis study was conducted in the lab of plant tissue culture of Date Palm Research Centre at the University of Basrah during the seasons 2010 and 2011, to determent of effect of different levels of sucrose in the growth and development of embryos physical for date palm variety of Barhi in vitro and content of protein carbohydrates and phenolic. The results showed that exposure of tissue cultured date palm tissues in vitro and growth under levels of 30 g / L sucrose led to the increase in fresh weight of embryos as reflected positively on the rate of biological processes where the embryos showed a significant increased in the protein content of different concentrations compared to the other. The results also show increased content of embryogenesis grown in various medium of carbohydrates with increasing concentration of sucrose from 10-50 g / L, where log concentration 50 g / L from the highest to the content of embryos of sugars, which amounted to (2.35) mg / g fresh weight and the difference was not significant for the content of embryos grown in the medium supplied with 30 g / L of sucrose. While the medium showed the supplied with 10 g / L of sucrose was significantly superior in reducing the content of the phenolic compounds in embryos, which amounted to (0.691) mg / g fresh weight compared with concentrations of 30 and 50 g / L of it. The study also showed the effects source of embryos grown at different concentrations of sucrose in the percentage of germination as it reached the highest percentage of germination (70%) where recorded for embryos originating from the medium supplied with 30 g / L sucrose, thus this treatment significant superiority compared with percentage of germination recorded for the embryos in concentrations of 50 and 10 g / L of it.
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